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Fig. 1 | BMC Research Notes

Fig. 1

From: Pseudomonas aeruginosa prioritizes detoxification of hydrogen peroxide over nitric oxide

Fig. 1

H2O2 clearance is prioritized over that of NO. P. aeruginosa cultures were grown to exponential phase and inoculated, at an OD600 of 0.025, into a bioreactor containing either 0, 10, or 20 μM H2O2. Immediately after addition of cells, cultures were treated with either 50 μM DPTA NONOate or the same volume of the DPTA NONOate solvent. a NO concentrations in the bioreactor were continuously measured. b Cumulative cellular NO consumption was assessed using a kinetic model with a black-box cellular compartment. c H2O2 concentrations were measured at 10-min intervals. d Culturability of P. aeruginosa in the presence of 50 μM DPTA and 50 μM DPTA + 20 μM H2O2 were assessed at the beginning and 1 h after treatment. All data represents the mean of three replicates, with error bars representing the standard error of the mean

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