Fig. 2

a Mice with combined knockout of Hdac1 and 2 (Cre+) show markedly reduced life span compared to wildtype littermates (Cre−). b At 16 weeks of age thymus, spleen and lymph nodes are strongly enlarged in knockout (Cre+) animals when compared to wildtype littermates (Cre−). c Histology and immunohistochemistry of 16 week old mice, comparing spleen samples of mice with combined Cx3cr1-dependent knockout of Hdac1 and 2 (Cre+) to their wildtype littermate (Cre−). Macrophages (Mac3), T-lymphocytes (CD3) and B-lymphocytes (B220) are depicted. Representative pictures of 5 Cre+ and 5 Cre−. Scale bar = 500 µm. d FACS analysis of cells isolated from thymus, spleen and lymph nodes from 16 week old Cre+ mice compared to their Cre− littermates. First column of each panel depicts gating for Thy1.2⁺ cells. The middle column shows CD4 vs CD8 fluorescence signal in the Thy1.2⁺ cells, while the right most column depicts CD24 vs Tcrβ fluorescence. Representative FACS plots for 5 replicates of each genotype are shown