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Table 1 Comparison of two studies [8, 9] and ddPCR-based expression changes for strain RO15, ScottA and S2542

From: The complete genome sequence of Listeria monocytogenes strain S2542 and expression of selected genes under high-pressure processing

(a) log2 fold change of the indicated gene after HPP at 400 MPa as determined by RNA-seq for RO15 and ScottA (Duru et al. [8]) or microarray for S2542 (Bowman et al. [9])

Gene

RO15 (RNA-seq, Duru et al. [8]) log2 fold change

ScottA (RNA-seq, Duru et al. [8]) log2 fold change

S2542

(microarray, Bowman et al. [9]) log2 fold change

agrB

− 0.35

− 0.15

2.84

hly

0.89

2.57

− 4.34

ftsE

− 1.11

− 0.66

2.71

clpE

2.26

2.27

− 3.93

mscL

0.24

0.33

1.04

Pearson correlation with S2542

− 0.87

− 0.99

 

(b) log2 fold change of the indicated gene immediately after HPP at 400 MPa as determined by ddPCR for all three strains (this study)

Gene

RO15

ScottA

S2542

agrB

− 0.10

− 0.16

− 0.32

hly

− 0.02

0.34

0.36

ftsE

− 0.30

− 0.06

0.20

clpE

0.27

0.27

0.57

mscL

0.01

− 0.73

0.83

Pearson correlation with S2542

0.49

− 0.24

 

(c) log2 fold change of the indicated gene immediately after HPP at 400 MPa and storage for 24 h as determined by ddPCR for all three strains (this study)

Gene

RO15

ScottA

S2542

agrB

0.36

0.09

0.59

hly

− 0.06

0.08

0.45

ftsE

0.02

0.60

1.78

clpE

0.28

0.21

0.35

mscL

0.57

0.82

2.30

Pearson correlation with S2542

0.38

0.97

 
  1. a log2 fold change results of the two different studies [8, 9] with samples treated at 400 MPa and correlation of the RNAseq results to the DNA microarray results. b ddPCR log2 fold change results at 0 min after 400 MPa treatment comparison with data from strains RO15, ScottA and S2542 showing the correlation of the gene expression between them. c ddPCR log2 fold change results at 24 h after 400 MPa treatment with data from strains RO15, ScottA, and S2542 showing the correlation of the gene expression between them