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Table 1 LC–MS data obtained from the glucose-associated L. agilis transcriptome in the absence of rRNA

From: Epitranscriptomic profile of Lactobacillus agilis and its adaptation to growth on inulin

Ribonucleoside detected and its short name Retention timea (min) Measured massb (Da) Mass accuracyc (ppm)
Cytidine, C 0.90 244.0935 0.7
Dihydrouridine, D 0.94 247.0933 3.3
Pseudouridine, Y 0.98 245.0775 3.0
1-Methyladenosine, m1A 1.55 282.1205 3.1
5-Methylcytidine, m5C 1.63 258.1093 3.2
Uridine, U 2.00 245.0776 3.3
7-Methylguanosine, m7G 2.54 298.1155 3.1
2′-O-Methylcytidine, Cm 2.79 258.1093 3.2
2′-O-Methylpseudouridine, Ym 2.99 259.0934 3.5
Guanosine, G 3.99 284.0997 2.8
5-Methyluridine, m5U 4.06 259.0933 3.2
Adenosine, A 4.45 268.1048 2.8
3-Methyluridine, m3U 4.86 259.0933 3.1
1-Methylguanosine, m1G 5.27 298.1155 3.2
2′-O-Methylguanosine, Gm 5.27 298.1155 3.2
N2-methylguanosine, m2G 5.53 298.1155 3.2
N4-acetylcytidine, ac4C 5.61 286.1034 0.2
2′-O-Methyladenosine, Am 5.64 282.1204 2.8
N6-methyladenosine, m6A 6.31 282.1206 3.2
N6,N6-dimethyladenosine, m6,6A 7.93 296.1354 0.2
N6-threonylcarbamoyladenosine, t6A 8.04 413.1417 0.8
  1. a ± 0.01 min
  2. bMass of protonated precursor ion
  3. cReference to the monoisotopic mass of protonated precursor ion