Fig. 2

a The assessment of cell-free supernatant effect on the TLRs genes in the IBD model by Real Time PCR based on SYBR Green method. P < 0.01 were considered statistically significant; b TLRs recruit different adaptors like as MyD88, TRIF, TIRAP/MAL, or TRAM. All TLRs can utilize the Myd-88 adaptor and activates NF-kB and MAPKs for induction of inflammatory cytokines. Key genes in this pathway which were evaluated in the study showed by arrows; c The evaluation of key mediator expression genes in TLR signaling pathway in present of cell-free supernatant. P < 0.01 were considered statistically significant