Evaluation of E. coli Nissle1917 derived metabolites in modulating key mediator genes of the TLR signaling pathway

Table 1 Sequence of primers used in qPCR in cell line

Genes Name	Forward Sequence (5′-3′)	Reverse Sequence (5′-3′)	Refs.
TLR-1	GCCCAAGGAAAAGAGCAAAC	AAGCAGCAATATCAACAGGAG	[43]
TLR-2	TCTCCCATTTCCGTCTTTTT	GGTCTTGGTGTTCATTATCTTC	[43]
TLR-3	TAAACTGAACCATGCACTCT	TATGACGAAAGGCACCTATC	[18]
TLR-4	GAAGCTGGTGGCTGTGGA	GATGTAGAACCCGCAAG	[18]
TLR-5	TTGCTCAAACACCTGGACAC	CTGCTCACAAGACAAACGAT	[18]
TLR-6	GTGCCATTACGAACTCTA	TTGTTGGGAATGCTGTT	[18]
TLR-7	CTGACCACTGTCCCTGAG	AACCCACCAGACAAACCA	[18]
TLR-8	AACATCAGCAAGACCCAT	GACTCCTTCATTCTCCCT	[18]
TLR-9	CGCCAACGCCCTCAAGACA	GGCGCTTACATCTAGTATTTGC	[18]
Myd88	TGGCCTTGTTAGACCGTGA	AAGTATTTCTGGCAGTCCTCCTC	[44]
IRAK1	AGGTTTCGTCACCCAAACATT	CGGGCTGTACCCAGAAGGA	[45]
IRAK4	CCTGACTCCTCAAGTCCAGAA	ACAGAAATGGGTCGTTCATCAAA	[46]
MAPK	GACGAGGAGCTTATGGTTCTGT	TTTTCATCCACTGTTGACCGAA	[47]
NFkB	GTCAAAAACGCCACCTCTCAA	CTCGCATGGAATTTGGAACCG	[22]
TAB2	CTCCTGGTGGTACAACTCGAC	TGATTTGGCTGTTGAGATGAGG	[48]
IFN-β	CAACTTGCTTGGATTCCTACAAAG	TATTCAAGCCTCCCATTCAATTG	[48]
Β-actin	AACCGTGAAAAGATGACCCAGAT	CACAGCCTGGATGGCTACGT	[19]
GAPDH	AACGGGAAGCTTGTCATCAATGGAAA	GCATCAGCAGAGGGGGCAGAG	[18]

ISSN: 1756-0500