Fig. 1

Functional annotation pipeline for E. okinawaensis midgut transcriptome assembly. To analyze the E. okinawaensis midgut transcriptome, we annotated the translated peptide sequence set through sequential BLASTP using several model organisms and Manduca sexta protein sequences, and we then also annotated the translated peptide sequence set via sequential BLASTP using 71 species of protein sequences obtained from the Ensembl Metazoa database. A functional gene annotation pipeline was used for the comparative pathway and gene enrichment analyses of eight species of stick insects using KEGG or Metascape. The stick insect species are as follows: a Aretaon asperrimus (male); b Entoria okinawaensis (female); c Clitarchus hookeri (female); d Ramulus artemis (female); e Medauroidea extradentata (female); f Peruphasma schultei (female); g Extatosoma tiaratum (female); and h Sipyloidea sipylus (female). The sequencer and text image drawings are from TogoTV (^©2016 DBCLS TogoTV/CC-BY-4.0). Mr. Satoshi Goto from the Tabunoki laboratory gifted all image drawings of stick insects