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Fig. 2 | BMC Research Notes

Fig. 2

From: De novo transcriptome analysis for examination of the nutrition metabolic system related to the evolutionary process through which stick insects gain the ability of flight (Phasmatodea)

Fig. 2

Hierarchical clustering and Gene set enrichment analysis using Metascape of eight stick insect midgut transcriptomes. a Hierarchical clustering was performed using TIBCO Spotfire Desktop version 7.6.0. The heatmap is colored based on quartiles. In other words, the gene expression value was sorted in ascending order for each stick insect transcriptome. The first quartile was assigned the middle number between the smallest number and the median of the dataset (blue), and the third quartile was assigned the middle value between the median and the highest value of the dataset (yellow). Genes that did not correspond to E. okinawaensis are shown in gray as missing values. The pink-colored solid rectangles show the flightless-wingless species, the green-colored solid rectangles show the flight-winged species, and blue-colored dotted rectangles show the flightless-winged stick insect species. b Comparison between flight and flightless insects. c Comparison between winged and wingless insects. A bar graph of the enriched terms across genes with high expression in the flight or winged group is shown. The different color intensities indicate significance for the corresponding GO term

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