Fig. 1

Study outline. A Schematic representation of the ET-1 gene showing 5′ upstream regulatory elements (5′URE) and the five exons of edn1 gene represented by boxes E1 to E5; the amplified region of 622 bps (−288 to + 344 position) including proximal promoter region, 5′ UTR and exon 1 as shown were amplified using primers—forward primer: AGCTTGCAAAGGGGAAGC; reverse primer: ACCTGTTTCTGGAGCTCC) using edn1 gene reference sequence (locus NG_016196 on chromosome 6) available at NCBI [17]. B Outline of the study depicting the three parallel arms undertaken, namely, characterization of clinical samples (parallel study), gene sequencing and measurement of ET-1 levels by ELISA