Fig. 2

Kinetic analysis of mCherry-BAX recruitment in the presence of elamipretide. A A typical sigmoid BAX recruitment curve showing various metrics that were obtained, including the maximum rate and the times for initiation and end of BAX accumulation, which are used to determine the duration of the recruitment period. The plateau of the recruitment curve is used to determine the total accumulation of BAX being recruited. This curve represents individual BAX puntae, which are then averaged for the entire cell being examined. B Scatter plot of calculated maximal recruitment rates for cells analyzed in each treatment group. C Combined BAX recruitment data for vehicle (black circles) plotted against all elamipretide groups combined (grey circles). The data used in this analysis represents the first 20 min of recruitment for all cells that were successfully imaged and analyzed. The combined data for elamipretide shows a significantly slower rate of recruitment (P = 6.76e−5, paired t-test). D Duration periods for all treatment groups. Treatment with 0.01 µM elamipretide yielded significantly longer duration periods compared to vehicle treated cells (*P = 0.004). E Bar graph showing duration times of vehicle treated cells against the combined elamipretide treated groups. Durations are significantly longer in the presence of elamipretide (*P = 0.036). F Bar graph showing the total amount of BAX recruited in vehicle treated cells relative to the combined elamipretide treated groups. No significant difference was detected