Fig. 3

Analysis of apoptotic mitochondrial fragmentation in the presence of elamipretide. Scatter plot of average mitochondrial volume of ARPE-19 cells treated with vehicle or different concentrations of elamipretide. Mitochondrial volumes were calculated from time lapse confocal images of cells expressing mitoBFP to label mitochondria. Average volumes were calculated at least 20 min prior to the onset of mCherry-BAX recruitment (Phase 1), during the period of mCherry-BAX recruitment (Phase 2), and during a 20 min interval after mCherry-BAX recruitment (Phase 3) (see Fig. 2A). ANOVA analysis showed no difference in volumes among the treatment groups in each phase (P ≥ 0.38). All treatment groups showed a significant decrease in mitochondrial volume in Phase 3 relative to the other Phases (1-way ANOVA *P < 0.006)