Fig. 2

Disc assay using LC fractions 31–36 from diethyl ether extraction and LCMS data. A The LC protocol was run to collect fractions of the RIT452 diethyl ether extract. Each was condensed, fully dried, and re-suspended in 100 µl of methanol. 60 µl of each fraction was added to a sterile diffusion disc and plated on LB agar seeded with S. aureus. The zone of inhibition (ZOI) of fraction 32 is 15 mm after 16 h of growth. B The LC channel in the LCMS shows only one chromatographic peak, while the MS channel shows at least four ionizable species in the positive scan mode. C The MS pattern corresponding to the beginning of the largest MS peak (0.652 min) in B shows the peaks 335.5, 401.7 and 445.6 in the positive scan mode between 100 and 1000 m/z