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Fig. 3 | BMC Research Notes

Fig. 3

From: Development of a method to rapidly assess resistance/susceptibility of Micro-Tom tomatoes to Tomato yellow leaf curl virus via agroinoculation of cotyledons

Fig. 3

Analysis of DNA extracted from true leaves of Micro-Tom seedlings after inoculation. PCR products amplified from DNA extract with TYLCV-specific primers were analyzed on 2% agarose gel. a Analysis of DNA extracted from true leaves of Micro-Tom seedlings 10 days post-inoculation. Lane M: a DNA size marker (TrackIt 1 kb Plus DNA Ladder, Invitrogen), lane C: PCR product using pBS-TYLCV as a template, lane 1: PCR products from the first true leaf, lane 2: PCR products from the second true leaf, lane 3: PCR products from the third true leaf, lane 4: PCR products from the fourth true leaf. b Analysis of DNA extracted from third true leaves of Micro-Tom seedlings 4–10 days post-inoculation. Lane M: a DNA size marker (TrackIt 1 kb Plus DNA Ladder), lane C: PCR product using pBS-TYLCV as a template, lanes 17: PCR products from the third true leaves 4–10 days post-inoculation, respectively. TYLCV viral DNA was detected on the 6th day post-inoculation

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