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Fig. 1 | BMC Research Notes

Fig. 1

From: Archaeal Orc1 protein interacts with T-rich single-stranded DNA

Fig. 1

ApOrc1 protein binds preferentially bottom strand of the AT-rich region of Ori1. A DNA sequence of the top strand of A. pernix Ori1 region (chromosome sequence coordinates: 444,909–445,231). ApOrc1 binding sites (Origin Recognition Box, ORB) are marked as black underlined letters. The AT-rich region is located between the ORB2 and ORB3 binding sites and is divided into four fragments: left 1 (L1) marked in red, left 2 (L2) marked in magenta, right 1 (R1) marked in light green and right 2 (R2) marked in dark green. B The scheme of experiment with the use of bio-layer interferometry technique. In the analysis the BLItz system with the SA biosensor was used. Biotinylated ssDNA fragments (Additional file 1: Table S1) containing sequence of the AT-rich region of Ori1 were immobilized on biosensor surface and then incubated with the ApOrc1 protein (250 nM), followed by incubation with a EDBS buffer. C, D BLItz sensograms showing binding of the ApOrc1 protein to the indicated ssDNA fragments (B, step 3), followed by the protein’s dissociation (B, step 4). As a negative control, binding of ApOrc1 to the SA biosensor without ssDNA was analyzed (blue curve)

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