Fig. 2
From: Archaeal Orc1 protein interacts with T-rich single-stranded DNA
ApOrc1 binds with higher affinity to the bottom strand of the R fragment. Binding of the ApOrc1 protein to ssDNA fragments (Additional file 1: Table S1) containing right top (A) and bottom (B) strands’ sequence of the Ori1 AT-rich region was analyzed with SPR technique. Biotinylated ssDNA fragments were immobilized on a surface of SA sensor chip. Injections containing the indicated concentrations of protein in HBS-EP buffer supplemented with ATP were performed. HBS-EP was used as a running buffer