Fig. 1

Amplification with primers that amplify the ORF together with the border sequences upstream and downstream—6366 bp (FB_Mfu10_RSeq1) and part of the ORF alone—3539 bp (MVH_FB_Mfu10). 6366 bp fragment is amplified only in resistant MAL0045 and clone 46H22 but the 3539 bp appears to be amplified in both resistant and susceptible clones but not in the susceptible parent— ‘Idared’ (a). Similarly, the progeny with a resistant phenotype to Ea222—05210-147 amplifies the 6366 bp but not the progeny with a susceptible phenotype—05210-89 (b). Like the susceptible clone, 94B13, 05210-89 appears to amplify the 3539 bp (c). Sequencing of amplicons amplified by 05210-147 and 05210-89 revealed the 8 bp insertion (highlighted in red) present in susceptible genotypes (d). *MAL0045 = 40 ng of DNA used. 1 kb ladder used