Fig. 2

Proof that transgenic line M2117 is positive for the selectable marker gene nptII, and gene-specific marker (a). FB_Mfu10-RSeq-ORF primer amplifies part of the border sequence and part of the first exon. D34 plasmid DNA (construct plasmid DNA) and ‘Pinova’ serve as positive and negative controls, respectively. 1 kb ladder used. Sequencing of amplicon amplified by line M2117 and compared with the susceptible progeny 05210-89 shows the same characteristics as the sequence as the resistant 05210-147 (b) lacking the 8 bp insertion. M. fusca and M2117 cDNAs amplify only exons sequence—1104 bp using FR_FB_Mfu10 (c), which borders a 1577 bp region of the candidate gene