The hepatoprotective effects of Pyrus biossieriana buhse leaf extract on tert-butyl hydroperoxide toxicity in HepG2 cell line

Table 1 The effect of pyrus biossieriana buhse leaf extract and t-BHP treatment on the cell viability of HepG2 cell line

Control 100.0 ± 1.10		p value	t100 72.86 ± 2.10		p value	t150 63.82 ± 1.7		p value
E1.5	94.36 ± 2.56	0.016	t150	63.82 ± 1.7	< 0.001	t200	47.07 ± 1.91	< 0.001
E2.0	81.99 ± 2.07	< 0.001	E1.5	94.36 ± 2.56	< 0.001	E1.5	94.36 ± 2.56	< 0.001
E2.5	73.25 ± 1.81	< 0.001	E2.0	81.99 ± 2.07	< 0.001	E2.0	81.99 ± 2.07	< 0.001
t100	72.86 ± 2.10	< 0.001	E2.5	73.25 ± 1.81	0.856	E2.5	73.25 ± 1.81	< 0.001
t150	63.82 ± 1.7	< 0.001	t100 + E1.5	85.38 ± 7.43	< 0.001	t150 + E1.5	78.94 ± 1.98	< 0.001
t200	47.07 ± 1.91	< 0.001	t100 + E2.0	76.81 ± 1.83	0.084	t150 + E2.0	68.79 ± 1.46	0.032
t100 + E1.5	85.38 ± 7.43	< 0.001	t100 + E2.5	65.49 ± 2.62	0.002	t150 + E2.5	60.01 ± 2.58	0.096

E1.5 94.36 ± 2.56		p value	E2.0 81.99 ± 2.07		p value	E2.5 73.25 ± 1.81		p value
E2.0	81.99 ± 2.07	< 0.001	E2.5	73.25 ± 1.81	< 0.001	t100	72.86 ± 2.10	0.856
E2.5	73.25 ± 1.81	< 0.001	t100 + E2.0	76.81 ± 1.83	0.026	t100 + E2.5	65.49 ± 2.62	0.001
t100 + E1.5	85.38 ± 7.43	< 0.001	t150 + E2.0	68.79 ± 1.46	< 0.001	t150 + E2.5	60.01 ± 2.58	< 0.001
t150 + E1.5	78.94 ± 1.98	< 0.001	t200 + E2.0	51.94 ± 2.72	< 0.001	t200 + E2.5	43.48 ± 3.20	< 0.001
t200 + E1.5	72.52 ± 1.10	< 0.001	E1.5	94.36 ± 2.56	< 0.001	t200 + E2.0	51.94 ± 2.72	< 0.001

The experiment was repeated five times and the average is reported with standard error for each group. Statistical analysis was performed using one-way analysis of variance (ANOVA) with subsequent post hoc comparisons by POST HOC (LSD) TEST (SPSS 21.0). (unit of measurement = percentage)
t: tert-Butyl hydroperoxide (t-BHP) E: Pyrus biossieriana Buhse leaves extract
t 100 (t-BHP, concentration: 100 µM), t 150 (t-BHP, concentration: 150 µM), t 200 (t-BHP, concentration: 200 µM)

ISSN: 1756-0500