The hepatoprotective effects of Pyrus biossieriana buhse leaf extract on tert-butyl hydroperoxide toxicity in HepG2 cell line

Table 2 The effect of pyrus biossieriana buhse leaf extract and t-BHP treatment on nitrite production in HepG2 cell line

Control 28.20 ± 1.15		p value	t100 55 ± 0.97		p value	t150 56 ± 1.16		p value
E1.5	31 ± 3.33	0.090	t150	56 ± 1.16	0.573	t200	70 ± 5.40	< 0.001
E2.0	31.80 ± 1.1	0.020	E1.5	31 ± 3.33	< 0.001	E1.5	31 ± 3.33	< 0.001
E2.5	48.30 ± 1.90	< 0.001	E2.0	31.80 ± 1.1	< 0.001	E2.0	31.80 ± 1.1	< 0.001
t100	55 ± 0.97	< 0.001	E2.5	48.30 ± 1.90	< 0.001	E2.5	48.30 ± 1.90	< 0.001
t150	56 ± 1.16	< 0.001	t100 + E1.5	40 ± 2.63	< 0.001	t150 + E1.5	41 ± 0. 80	< 0.001
t200	70 ± 5.40	< 0.001	t100 + E2.0	43.7 ± 2.80	< 0.001	t150 + E2.0	47.40 ± 30	< 0.001
t100 + E1.5	40 ± 2.63	< 0.001	t100 + E2.5	59 ± 0.70	< 0.001	t150 + E2.5	60 ± 0.60	< 0.001

E1.5 31 ± 3.33		p value	E2.0 31.80 ± 1.1		p value	E 2.5 48.30 ± 1.90		p value
E2.0	31.80 1.1	0.507	E2.5	48.30 ± 1.90	< 0.001	t100	55 ± 0.97	< 0.001
E2.5	48.30 ± 1.90	< 0.001	t100 + E2.0	43.7 ± 2.80	< 0.001	t100 + E2.5	59 ± 0.70	< 0.001
t100 + E1.5	40 ± 2.63	< 0.001	t150 + E2.0	47.40 ± 30	< 0.001	t150 + E2.5	60 ± 0.60	< 0.001
t150 + E1.5	41 ± 0. 80	< 0.001	t200 + E2.0	50.20 ± 0.60	< 0.001	t200 + E2.5	65.6 ± 0.60	< 0.001
t200 + E1.5	42.5 ± 1.94	< 0.001	E1.5	31 ± 3.33	< 0.001	t200 + E2.0	50.20 ± 0.60	0.190

The experiment was repeated five times and the average is reported with standard error for each group. Statistical analysis was performed using one-way analysis of variance (ANOVA) with subsequent post hoc comparisons by POST HOC (LSD) TEST (SPSS 21.0). (unit of measurement = µM)
t: tert-Butyl hydroperoxide (t-BHP) E: Pyrus biossieriana Buhse leaves extract
t 100 (t-BHP, concentration: 100 µM), t 150 (t-BHP, concentration: 150 µM), t 200 (t-BHP, concentration: 200 µM)

ISSN: 1756-0500