The hepatoprotective effects of Pyrus biossieriana buhse leaf extract on tert-butyl hydroperoxide toxicity in HepG2 cell line

Table 3 The effect of pyrus biossieriana buhse leaf extract and t-BHP treatment on antioxidant capacity in HepG2 cell line

Control 180.10 ± 14.87		p value	t100 140 ± 12.69		p value	t150 96 ± 9.76		p value
E1.5	1814 ± 64.85	< 0.001	t150	96 ± 9.76	0.351	t200	80 ± 8.80	0.486
E2.0	2498 ± 37.83	< 0.001	E1.5	1814 ± 64.85	< 0.001	E1.5	1814 ± 64.85	< 0.001
E2.5	3952 ± 37.83	< 0.001	E2.0	2498 ± 37.83	< 0.001	E2.0	2498 ± 37.83	< 0.001
t100	140 ± 12.69	0.078	E2.5	3952 ± 37.83	< 0.001	E2.5	3952 ± 37.83	< 0.001
t150	96 ± 9.76	0.008	t100 + E1.5	1381 ± 57.96	< 0.001	t150 + E1.5	1081 ± 87.36	< 0.001
t200	80 ± 8.80	0.001	t100 + E2.0	2438 ± 32.23	< 0.001	t150 + E2.0	2294 ± 30.81	< 0.001
t100 + E1.5	1381 ± 57.96	< 0.001	t100 + E2.5	3832 ± 34.49	0.002	t150 + E2.5	3508 ± 23.93	0.096

E1.5 1814 ± 64.85		p value	E2.0 2498 ± 37.83		p value	E2.5 3952 ± 37.83		p value
E2.0	2498 ± 37.83	< 0.001	E2.5	3952 ± 37.83	< 0.001	t100	140 ± 12.69	< 0.001
E2.5	3952 ± 37.83	< 0.001	t100 + E2.0	2438 ± 32.23	0.03	t100 + E2.5	3832 ± 34.49	< 0.001
t100 + E1.5	1381 ± 57.96	< 0.001	t150 + E2.0	2294 ± 30.81	< 0.001	t150 + E2.5	3508 ± 23.93	< 0.001
t150 + E1.5	1081 ± 87.36	< 0.001	t200 + E2.0	2234 ± 12.14	< 0.001	t200 + E2.5	3075 ± 97.91	< 0.001
t200 + E1.5	924.9 ± 33.78	< 0.001	E1.5	1814 ± 64.85	< 0.001	t200 + E2.0	2234 ± 12.14	< 0.001

The experiment was repeated five times and the average is reported with standard error for each group. Statistical analysis was performed using one-way analysis of variance (ANOVA) with subsequent post hoc comparisons by POST HOC (LSD) TEST (SPSS 21.0). (unit of measurement = µM)
t: tert-Butyl hydroperoxide (t-BHP) E: Pyrus biossieriana Buhse leaves extract
t 100 (t-BHP, concentration: 100 µM), t 150 (t-BHP, concentration: 150 µM), t 200 (t-BHP, concentration: 200 µM)

ISSN: 1756-0500