The hepatoprotective effects of Pyrus biossieriana buhse leaf extract on tert-butyl hydroperoxide toxicity in HepG2 cell line

Table 4 The effect of pyrus biossieriana buhse leaf extract and t-BHP treatment on TBA equivalent (lipid peroxidation) in HepG2 cell line

Control 1.30 ± 0.04		p value	t100 3.17 ± 0.58		p value	t150 3.86 ± 0.06		p value
E1.5	1.28 ± 0.06	0.008	t150	3.86 ± 0.06	< 0.001	t200	5.9 ± 0.18	< 0.001
E2.0	2.44 ± 0. 03	< 0.001	E1.5	1.28 ± 0.06	< 0.001	E1.5	1.28 ± 0.06	< 0.001
E2.5	3.16 ± 0.07	< 0.001	E2.0	2.44 ± 0. 03	< 0.001	E2.0	2.44 ± 0. 03	< 0.001
t100	3.17 ± 0.58	< 0.001	E2.5	3.16 ± 0.07	< 0.001	E2.5	3.16 ± 0.07	< 0.001
t150	3.86 ± 0.06	< 0.001	t100 + E1.5	2.26 ± 0.03	< 0.001	t150 + E1.5	2.72 ± 0.07	< 0.001
t200	5.91 ± 0.18	< 0.001	t100 + E2.0	2.86 ± 0.03	< 0.001	t150 + E2.0	3.01 ± 0.05	< 0.001
t100 + E1.5	2.26 ± 0.03	< 0.001	t100 + E2.5	3.91 ± 0.07	< 0.001	t150 + E2.5	5.72 ± 0. 10	< 0.001

E1.5 1.28 ± 0.06		p value	E2.0 2.44 ± 0. 03		p value	E2.5 3.16 ± 0.07		p value
E2.0	2.44 ± 0. 03	< 0.001	E2.5	3.16 ± 0.07	< 0.001	t100	3.17 ± 0.58	< 0.001
E2.5	3.16 ± 0.07	< 0.001	t100 + E2.0	2.86 ± 0.03	< 0.001	t100 + E2.5	3.91 ± 0.07	< 0.001
t100 + E1.5	2.26 ± 0.03	< 0.001	t150 + E2.0	3.01 ± 0.05	< 0.001	t150 + E2.5	5.72 ± 0. 10	< 0.001
t150 + E1.5	2.72 ± 0.07	< 0.001	t200 + E2.0	3.96 ± 0.14	< 0.001	t200 + E2.5	6.23 ± 1.36	< 0.001
t200 + E1.5	3.76 ± 0.09	< 0.001	E1.5	1.28 ± 0.06	< 0.001	t200 + E2.0	3.96 ± 0.14	< 0.001

The experiment was repeated five times and the average is reported with standard error for each group. Statistical analysis was performed using one-way analysis of variance (ANOVA) with subsequent post hoc comparisons by POST HOC (LSD) TEST (SPSS 21.0). (unit of measurement = µM)
t: tert-Butyl hydroperoxide (t-BHP) E: Pyrus biossieriana Buhse leaves extract
t 100 (t-BHP, concentration: 100 µM), t 150 (t-BHP, concentration: 150 µM), t 200 (t-BHP, concentration: 200 µM)

ISSN: 1756-0500