Optimization of a suite of flathead catfish (Pylodictis olivaris) microsatellite markers for understanding the population genetics of introduced populations in the northeast United States

White, Shannon L.; Eackles, Michael S.; Wagner, Tyler; Schall, Megan; Smith, Geoff; Avery, Julian; Kazyak, David C.

doi:10.1186/s13104-021-05725-2

Table 1 Characteristics of 13 microsatellite loci tested for genetic analyses of flathead catfish (Pylodictis olivaris)

From: Optimization of a suite of flathead catfish (Pylodictis olivaris) microsatellite markers for understanding the population genetics of introduced populations in the northeast United States

Locus	Primer Sequences 5′-3′	Multiplex mix	Fluorescent dye	μM	Size range (bp)	Repeat motif	Source
GY113J02	F: CACGTTCAGGCCAATACAACAC	B	6-FAM	0.3	370–400	AAT₍₁₇₎	[11]
GY113J02	R: GTTTGTCCACTACCTTGTGCCC	B	6-FAM	0.3	370–400	AAT₍₁₇₎
GY047K03	F: CCCTCTATGCCTGTGATTGTTTATG	B	6-FAM	0.2	190–230	Not available	[11]
GY047K03	R: GTTTGTCCACCAAGTCCCTGTGTAAC	B	6-FAM	0.2	190–230	Not available	[11]
IpCG0071	F: CGAAGGTTTATAACTAAGGAGCAGG	B	VIC	0.1	145–190	AGAT₍₁₁₎GGAT₍₃₎AGAT₍₂₎ GGAT₍₆₎[AGAT₍₁₎GGAT₍₃₎]₄	[11]
IpCG0071	R: GTTTGTACCTGGCTGTGAAGACAC	B	VIC	0.1	145–190	AGAT₍₁₁₎GGAT₍₃₎AGAT₍₂₎ GGAT₍₆₎[AGAT₍₁₎GGAT₍₃₎]₄	[11]
71–75	F: CGCTAAATGATCCATTCCACC	C	PET	0.2	110–160	CA₍₁₂₎	[11]
71–75	R: GTTTCAGTGCCGCCATTCTCAC	C	PET	0.2	110–160	CA₍₁₂₎	[11]
Ip077	F: GAAACACAATGTACAGTAAGCTG	C	6-FAM	0.2	110–150	GT	[14]
Ip077	R: GCTGCTTCTTATGGAATCTC	C	6-FAM	0.2	110–150	GT	[14]
IpCG00189	F: GATCCTGTGCTAAAGAAACCAAG	A	VIC	0.1	200–300	AAT	[12, 13]
IpCG00189	R: GTGCCGCAGTGTGTTGTAAA	A	VIC	0.1	200–300	AAT	[12, 13]
Ip271	F: TGGGGAAAAAGAAAGTAATAACG	A	6-FAM	0.2	155–185	TG₍₁₈₎GA₍₁₉₎	[10]
Ip271	R: CAGTAGAGCTTTGAAGCCATTC	A	6-FAM	0.2	155–185	TG₍₁₈₎GA₍₁₉₎	[10]
Ip357	F: CCTGCCACCATATCAGTGAATTT	A	NED	0.2	100–160	CA₍₁₈₎	[10]
Ip357	R: GATAATGAGTCTCCGGAGGTGC	A	NED	0.2	100–160	CA₍₁₈₎	[10]
Ip365	F: TAAAGGATCTGATTCACCGTATC	B	PET	0.3	100–145	CA₍₁₃₎	[10]
Ip365	R: AAACCGCTAACCTACCCTCT	B	PET	0.3	100–145	CA₍₁₃₎	[10]
Ip372	F: GGCACTGAGGTTTGGGCTGCAC	A	PET	0.3	190–220	CA₍₈₎	[10]
Ip372	R: TGGCATCGCTCCTCATCATCCTG	A	PET	0.3	190–220	CA₍₈₎	[10]
Ip554	F: GAGATGAAGTGAGATGAAGACAG	A	NED	0.2	220–255	GA₍₂₀₎	[10]
Ip554	R: TGCTTAAATAATGACACGGTTC	A	NED	0.2	220–255	GA₍₂₀₎	[10]
Ip591	F: CTGCTTTAGGTCCACCCACTGC	C	NED	0.2	130–180	GT, GA	[14]
Ip591	R: AGGCACTTGACATTTAGCCTGC	C	NED	0.2	130–180	GT, GA	[14]
IpCG0001	F: GTACCACTGGTCAGTATCTCC	C	VIC	0.1	175–215	AAT₍₂₀₎	[11]
IpCG0001	R: GTTTCACCATCACCAGAGTCCAGG	C	VIC	0.1	175–215	AAT₍₂₀₎	[11]

F Forward primer, R: reverse primer, Multiplex Mix assignment of each locus to one of three PCR reactions, Fluorescent Dye dye used for each locus within each PCR reaction, μM primer concentration used in the PCR reaction, Size Range (bp) bp size of the alleles genotyped, Repeat Motif motif and number of repeats (in parentheses) for each locus, Source citation for the locus

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ISSN: 1756-0500

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