Optimization of a suite of flathead catfish (Pylodictis olivaris) microsatellite markers for understanding the population genetics of introduced populations in the northeast United States

Table 2 Population genetics summary for four collections of flathead catfish (Pylodictis olivaris)

Site	N	A_R	rA_R	H_O	H_E	uH_E	F	P	% Poly Loci	HW # Sig Loci	LD # Sig Pairs	Average Pairwise \({F}_{ST}^{^{\prime}}\)
Schuylkill	43	3.77	3.51	0.50	0.51	0.51	-0.01	17	100%	1	2	0.31
Lower Susquehanna	48	2.54	2.54	0.49	0.50	0.51	0.03	0	100%	1	0	0.19
Middle Susquehanna	30	2.54	2.54	0.48	0.50	0.51	0.03	0	100%	0	0	0.14
Upper Susquehanna	16	2.62	2.62*	0.60	0.51	0.53	-0.19	2	100%	0	0	0.20

N, sample size; A_R, allelic richness; rA_R, rarefied allelic richness (standardized to 40 alleles); H_O, observed heterozygosity; H_E, expected heterozygosity; uH_E, unbiased expected heterozygosity; F, fixation index; P, number of private alleles; % Poly Loci, percent of loci that were polymorphic; HW # Sig Loci, number of loci with significant deviation from HWE; LD # Sig Pairs, number of loci pairs with significant linkage disequilibrium; Average pairwise \({F}_{ST}^{^{\prime}}\), average \({F}_{ST}^{^{\prime}}\) across all pairwise comparisons
* Denotes a rarefied allelic richness value standardized to a larger number of alleles (40) than were sampled in the collection (32) and so the value assumes all alleles were present in the sample

ISSN: 1756-0500