Fig. 1
From: Rapid spheroid assays in a 3-dimensional cell culture chip
The 3D cell culture chip. a The design of the 3D cell culture chip (AIM Biotech). The chip is designed as a standard size microscope specimen slide and it consists of three columns. Each column comprises of a gel channel surrounded by two media channels. Spheroids were passed through the gel channels after resuspending the spheroids with collagen. Media channels were filled with either complete media or complete media plus drugs depending on the objectives of the experiment. b Suspension of collagen matrix with spheroids into the gel channels of the 3D chip. c Hydrating the media channel ports with media only or media + drug. d Replenishment of media or media + drug in the chip