Skip to main content
Fig. 2 | BMC Research Notes

Fig. 2

From: Ratio between Lactobacillus plantarum and Acetobacter pomorum on the surface of Drosophila melanogaster adult flies depends on cuticle melanisation

Fig. 2

The ratio between L. plantarum and A. pomorum depends on the fly genotype. Applying qPCR, we detected L. plantarum and A. pomorum in the wash solution of fly surfaces in independent experiments using genus-specific primers (1–3). In ebony mutant flies (ebonycc1. 3 & 4) that derive from Tübingen2018 flies (Tü 2018) by gene editing, the relative fold-difference (y-axis) between L. plantarum and A. pomorum is reversed compared to the situation in the original flies. Data are shown as boxplots (n = 5 for Tübingen2018. n = 6 for ebony flies). Central traits represent the median, crosses the mean. Boxes indicate first and third quartile and whiskers represent the range. The broad data range is probably due to the genetic variation of the fly populations that derive from several founders. Data were analysed using the non-parametric Mann–Whitney U-test with the null hypothesis (H0) that the two populations “Tübingen2018” and “ebony” are not different. With α = 0.05 and U = 30. The p-value for this test is 0.004 allowing to refute H0. Applying the parametric Student’s T-test, we obtain a p-value of 0.013 (data now shown) suggesting a significant difference between the data of the two populations. We consider this test, however, as inappropriate as we cannot assume a normal distribution of the data

Back to article page