Fig. 3

Evaluation of CUT&RUN enrichment profiles with clustered signal heatmap. A Heatmap showing the normalized (reads per million (RPM) signal of each CUT&RUN dataset over consensus peak regions for A Ikaros replicate datasets (frozen) with IgG controls, or B H3K4me3 replicate datasets (frozen) with IgG controls. Clustering was performed with kmeans = 6 for both datasets, and shows an overall similar distribution of signal for called peaks across replicates, exhibiting variable signal across clusters. Also, in either dataset, cluster 1 regions show strong signal in IgG controls and are likely artifacts. These peaks do not map to known blacklisted regions but are associated with heterochromatin. These peaks should be filtered to obtain a final dataset