Table 1 Mapped read ratios of one-step PCR amplicons from cell-free DNA of RhD-negative pregnant women
Cell-free DNA from a RhD-negative pregnant woman | Amplicons from Rhesus boxes (Primers: RHbox_Tailed_F/R) | Amplicons from RHD/RHCE exon 9 (Primers: RHD/RHCE_exon9_Tailed_F/R) | |
|---|---|---|---|
Observed ratio and read counts (upstream/downstream) | Observed ratio and read counts (RHD_wt/RHD_var/RHCE) | ||
Cell-free DNA #46 (8 weeks) | 1.88%/98.12% (416/21,694) | 1.33%/0%/98.67% (281/0/20,914) | |
Cell-free DNA #59 (28 weeks) | 3.60%/96.40% (813/21,754) | 2.05%/0.002%/97.95% (1,253/1/59,758) | |