Fig. 1

Mutation rate (a) and mutation efficiency (b) of blastocysts derived from zona pellucida (ZP)-free zygotes subjected to lipofection mediated gene editing with gRNA targeting GGTA1. Zygotes were collected at 5 h, 10 h, or 15 h from the start of in vitro fertilization and incubated with Lipofectamine 2000, gRNA, and Cas9 protein for 5 h. a Mutation rate was defined as the ratio of the number of edited blastocysts to the total number of sequenced blastocysts. b Mutation efficiency was estimated as the proportion of the indel mutation events in gene-edited blastocysts. Numbers within parentheses indicate the total number of blastocysts examined. Each bar represents the mean ± SEM