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Table 1 Development of zona pellucida (ZP)-free zygotes subjected to lipofection-mediated gene editing with gRNA targeting GGTA1 for various incubation durations

From: Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events

Incubation duration* No. of zygotes examined No. (%) of zygotes developed to blastocysts
0 h (Control) 226 34 (15.1 ± 1.9)a
2.5 h 218 34 (15.5 ± 2.2)a
5 h 217 26 (11.7 ± 2.2)a
10 h 215 28 (12.8 ± 3.9)a
20 h 223 7 ( 3.0 ± 1.9)b
  1. All experiments were repeated five times. Percentage data are expressed as means ± SEM
  2. *ZP-free zygotes were collected at 10 h from the start of IVF and exposed to Lipofectamine 2000 for 0 h (Control), 2.5 h, 5 h, 10 h, or 20 h and then cultured for 7 days
  3. a,b Values with different superscript letters in the same column are significantly different (p < 0.05)