Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events

BMC Research Notes

Table 1 Development of zona pellucida (ZP)-free zygotes subjected to lipofection-mediated gene editing with gRNA targeting GGTA1 for various incubation durations

Incubation duration*	No. of zygotes examined	No. (%) of zygotes developed to blastocysts
0 h (Control)	226	34 (15.1 ± 1.9)^a
2.5 h	218	34 (15.5 ± 2.2)^a
5 h	217	26 (11.7 ± 2.2)^a
10 h	215	28 (12.8 ± 3.9)^a
20 h	223	7 ( 3.0 ± 1.9)^b

All experiments were repeated five times. Percentage data are expressed as means ± SEM
*ZP-free zygotes were collected at 10 h from the start of IVF and exposed to Lipofectamine 2000 for 0 h (Control), 2.5 h, 5 h, 10 h, or 20 h and then cultured for 7 days
^a,b Values with different superscript letters in the same column are significantly different (p < 0.05)

ISSN: 1756-0500