Fig. 1

Michaelis Menten plots of kinetics data obtained using CMP-Glo with hydrolyzed serogroup W acceptor and corresponding standard curves. A Data with varying acceptor (0–2000 µg/mL) and other components held constant. K_m and V_max determined to be 629.2 ± 101.4 µg/mL and a V_max of 0.8965 ± 0.05823 µM/min. B Data with varying CMP-NeuNAc (0–80 µM) and other components held constant. K_m and V_max were determined to be 13.84 ± 9.675 µM and 0.6205 ± 0.1331 µM/min. C Standard Curve for panel A. D Standard Curve for panel B. (These are one representative example of three individual experiments. All experiments were run with two replicates. Data point indicates the mean and error bars represent standard deviation)