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Fig. 1 | BMC Research Notes

Fig. 1

From: RT-QuIC detection of pathological prion protein in subclinical goats following experimental oral transmission of L-type BSE

Fig. 1

A, B PrPsc detection from subclinical goats following experimental oral transmission of L-type BSE. rHaPrPSen 90-231 substrate was used to detect PrPL-BSE from both brainstem A and pooled CNS B homogenates. Normal control brain homogenates (NBH, black) showed no response. Goat ID and dilutions are indicated next to the curve. C-D) Detection of PrPsc in different CNS areas of L-BSE infected goats at the asymptomatic stage using RT-QuIC assay. rHaSPrPSen 23-231 substrate was used at 55 °C to detect PrPL-BSE from different CNS areas collected from C G1 and D G2 goats. Each ThT reading is indicated as the percentage of the maximum value achievable by the plate readers as a function of reaction time

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