Fig. 2
RT-QuIC detection of PrPsc in different lympho nodes (A submandibular, B mesenteric, C mediastinal and D retropharyngeal) of L-BSE infected goats. Normal control tissue homogenates (black) showed no response. Goat ID are indicated next to the curve. RT-QuIC reactions were seeded with 2 μl of 10–4 LNs homogenate dilution and rHaPrPSen 29-231 substrate was used at 42 °C to detect PrPL-BSE. Each ThT reading is indicated as the percentage of the maximum value achievable by the plate readers as a function of reaction time. The positive threshold was calculated as 10% fluorescence increases of tissue homogenate from normal controls