Fig. 2

Centriolin is effectively targeted with the centriolin guide RNA and Cas9 in Hela cells. A Western blot of whole cell lysates from Hela cells transfected with either the centriolin guide RNA (gRNA)-Cas9 plasmid or empty Cas9 vector and probed with centriolin antibody. Alpha tubulin was used as a loading control. B Immunofluorescence staining of centriolin (red) and microtubules (MT, green), and DAPI staining of DNA in centriolin gRNA-treated Hela cells and empty vector controls. Note the brightly stained red dots of centriolin representing the centrosome in the empty vector panel, which are absent in the centriolin gRNA sample. Images were taken at 10× magnification