Table 1 Characteristics of 16 primer pairs for microsatellites loci developed in A. heterocarpa and A. sylvestris
Locus | Primer sequences (5ā²āāā3ā²) | Motif | Range (bp) | Label | Multiplex | GenBank | |
|---|---|---|---|---|---|---|---|
An23 | F: | GAAACAAAATCAAATAGTAATCGCA | AC | 75ā95 | PET | 1 | MZ065562 |
| Ā | R: | AAATGTAATCTGCACGCGGT | Ā | Ā | Ā | Ā | Ā |
An35 | F: | GGTTGCAACTCAGATGCTGG | GT | 175ā189 | FAM | 1 | MZ065564 |
| Ā | R: | ACCCTGTGCGTATTGCCTAC | Ā | Ā | Ā | Ā | Ā |
An69 | F: | AGCAAGTGAGGCAAGACCAT | AG | 144ā176 | FAM | 2 | MZ065568 |
| Ā | R: | CAAACTCTCCTTCACCCCAA | Ā | Ā | Ā | Ā | Ā |
An71 | F: | GAGCATCCTCGAAGAGATCAA | AG | 216ā262 | FAM | 1,2 | MZ065569 |
| Ā | R: | GGGACCACTCAGAATTGGAA | Ā | Ā | Ā | Ā | Ā |
An72 | F: | TTGGATTCAGGAGAGGACCA | GAT | 66ā114 | FAM | 2 | MZ065570 |
| Ā | R: | CTTCTCCCAACCAGGATCAG | Ā | Ā | Ā | Ā | Ā |
An74 | F: | GCATTGTAGCCTACTGGAGGG | GT | 80ā134 | VIC | 2 | MZ065571 |
| Ā | R: | GGCAACAGAGTAGACCTTAACCTG | Ā | Ā | Ā | Ā | Ā |
An89 | F: | GCAACCGCAGCTCATTTTAT | GT | 88ā106 | PET | 2 | MZ065572 |
| Ā | R: | AAACAAAGGACCAGCCGAC | Ā | Ā | Ā | Ā | Ā |
An91 | F: | CTCGCGTTGACAGGGTTTA | GT | 129ā143 | PET | 2 | MZ065573 |
| Ā | R: | TCGAGTTCTAGTTGGACAGGG | Ā | Ā | Ā | Ā | Ā |
An93 | F: | GGCAGCTAAGTGAAGCAAAA | AC | 170ā190 | VIC | 2 | MZ065574 |
| Ā | R: | TGCGCATGTTACTAAGGCTG | Ā | Ā | Ā | Ā | Ā |
S216 | F: | TCTCTGAGTATATATTTTTGGGTGTG | CT | 167ā179 | PET | 1 | MZ065559 |
| Ā | R: | TCGCAAAATACCCTCATCTC | Ā | Ā | Ā | Ā | Ā |
An11 | F: | GGGACATTCAACACAACATCA | AG | 96ā128 | VIC | 1 | MZ065561 |
| Ā | R: | CTCTTTCTTGCACCCTTCCA | Ā | Ā | Ā | Ā | Ā |
An39 | F: | TTGGCTGCACTTACATTTGC | CT | 212ā284 | VIC | 1 | MZ065565 |
| Ā | R: | ATGATAAACCCGGTTGCTTG | Ā | Ā | Ā | Ā | Ā |
An68 | F: | TCCAAAATGCACAGATCCAG | AG | 129ā171 | NED | 2 | MZ065567 |
| Ā | R: | CTCGTCGAGTTCTACGCTCC | Ā | Ā | Ā | Ā | Ā |
An32 | F: | TGGGTTCATCAAGATTCAAGG | AG | 118ā174 | NED | 1 | MZ065563 |
| Ā | R: | GTGTGGTCACTGCAAGCATC | Ā | Ā | Ā | Ā | Ā |
An41 | F: | GGGAAACTGAATTAACCGAGC | AG | 263ā324 | PET | 1 | MZ065566 |
| Ā | R: | CCACTTGTGGTCTCTAACATGG | Ā | Ā | Ā | Ā | Ā |
S37 | F: | CAAAAGTGGATACTAGTTGTGTG | CT | 192ā224 | NED | 1 | MZ065560 |
| Ā | R: | GCTCTACCATTAGCAAAACC | Ā | Ā | Ā | Ā | Ā |