Fig. 1
From: Assessment of source material for human intestinal organoid culture for research and clinical use
Composition of hIOs derived from transverse and sigmoid colon. hIOs derived from transverse colon (TC) and sigmoid colon (SC) biopsies (n = 3) from non-IBD donors were expanded over 12 days under standard media (SM). Epithelial composition was evaluated on day 12 by RT-qPCR. A. Scheme depicting the overall experiment. B. Principal component analysis (PCA) from expression of epithelial cells of TC- and SC hIO. C. Relative gene expression (2−ΔCT) of epithelial surface marks, crypt markers, and epithelial cell marker between TC- and SC hIO