Fig. 2
From: Assessment of source material for human intestinal organoid culture for research and clinical use
Comparison analysis of epithelial composition between organoids derived from fresh versus frozen biopsies. hIO were grown for 20 d from fresh and frozen SC biopsies (1 month at liquid nitrogen) (n = 6). Epithelial composition was evaluated on days 15 and 20 by RT-qPCR. A. Scheme depicting the overall experiment. B. PCA from RT-qPCR relative gene expression (2−ΔCT) data freshhIO (day 15) and frozenhIO (day 15,20). C. Relative gene expression between freshhIO (day 15) and frozenhIO (day 15,20). ANOVA one-way followed by Sidak’s multiple comparisons test were performed.* p < 0.05, *** p < 0.001, ns = not significant