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Fig. 2 | BMC Research Notes

Fig. 2

From: Selection and validation of reference gene for RT-qPCR studies in co-culture system of mouse cementoblasts and periodontal ligament cells

Fig. 2

A Schematic representation of the co-culture system used in the experiment with SV-PDL cells plated on the upper insert and OCCM-30 cells in the lower well. B, D, F The stability of top four reference genes was assessed by geNorm (n = 15) for both the direct cell–cell contact culture system (B) and the co-culture system (D, F). Lower M value predicts higher stability. C, E, G The suitable number of reference genes was determined by geNorm for bothe direct cell–cell contact culture system (C) and the co-culture system (E, G). The value of V less than the recommended cut-off of 0.15 is attained with two reference genes

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BMC Research Notes

ISSN: 1756-0500

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