Fig. 1

FLX treatment decreased the number of proliferating cells in the hypothalamus of adult mice. A, B Representative images of the NSC marker, nestin, positive structures in the hypothalamus of mice treated with vehicle (upper) or FLX (lower). C The fluorescence intensities of nestin-positive structure were quantified in the hypothalamus of vehicle-treated and FLX-treated mice (n = 4 mice each). D, E Representative images of the dividing cell marker, Ki67, positive cells in the hypothalamus of mice treated with vehicle (upper) or FLX (lower). F The number of Ki67-positive cells was quantified in the hypothalamus of vehicle-treated and FLX-treated mice (n = 4 mice each). Scale bar, 100 μm in A and B, 20 μm in D and E. 3V third ventricle; ME medial eminence. Values (mean ± SD) are analyzed by Student’s t test