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Fig. 1 | BMC Research Notes

Fig. 1

From: Using ZINC08918027 inhibitor to determine Aurora kinase-chromosomal passenger complex isoforms in mouse oocytes

Fig. 1

The ZINC08918027 (ZC) binding region is conserved, and the inhibitor perturbs meiotic progression in mouse oocytes. A Protein alignment of Xenopus AURKB and mouse AURKA/B/C. Yellow and green highlighted text indicate conserved residues and conservative changes in the ZC binding region respectively. The blue highlighted residues determine whether the AURK has affinity for INCENP (asparagine, N) or TPX2 (glycine, G). B Prophase I-arrested mouse oocytes were treated with the indicated doses of ZC and matured in vitro for 16 h, followed by detection of spindle (α-tubulin, green), kinetochores (anti-centromere antibody (ACA), red), and DNA (DAPI, blue). Met II progression was marked by polar body extrusion. The panels show representative images of metaphase I (Met I) arrest (PB −) and progression to metaphase II (Met II) (PB +). White circle outlines the oocyte and extruded polar bodies. Scale bar = 10 μm. C Percent of oocytes in each treatment that matured to the Met II stage. Error bars show standard error of the mean. D Quantification of Met II spindle length in each treatment. Data from three independent experiments with an average number of 25 Prophase I oocytes used/treatment/replicate (***p ≤ 0.001, One-way ANOVA)

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