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Fig. 1 | BMC Research Notes

Fig. 1

From: Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts

Fig. 1

Effects of ERN on osteoclast formation in BMCs. A Chemical structure of ERN. B Effect of ERN on the cell viability of BMCs. The cytotoxic effect of ERN was evaluated using the CCK-8 assay. BMCs were treated with various concentrations of ERN (0–5 µM) for 6 days. Cell viability is expressed as a percentage of the values obtained for untreated ERN-cells. C BMCs were cultured with various concentrations of ERN (0–1 µM) in the presence of 1α,25(OH)2D3 (10–8 M) for 6 days. After incubation, the cells were fixed and stained for TRAP, a marker enzyme for osteoclast differentiation. TRAP-positive multinuclear cells (≥ 3 nuclei) were counted. The effect of ERN on osteoclast differentiation is expressed as the rate of multinucleated osteoclast formation, with ERN-untreated cells set at 100%. The data are expressed as the means ± SE of three independent experiments (n = 3). Means marked with different letters are significantly different (P < 0.05)

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