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Fig. 3 | BMC Research Notes

Fig. 3

From: Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts

Fig. 3

Effects of ERN on osteoblast differentiation in BMCs. A Mineralization of BMCs was assessed by alizarin red staining after 15 days of culture with osteoblast-inducer reagents (ascorbic acid, β-glycerophosphate, and hydrocortisone). B Staining activities were quantified by measure optical absorbance at 570 nm using a microplate reader. The effect of ERN on osteoblast differentiation is expressed as the degree of mineralization, with ERN-untreated cells set at 100%. The data are expressed as the means ± SE of multiple independent experiments (n = 6). Means marked with different letters are significantly different (P < 0.05)

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