Fig. 2From: Structure-based design and construction of a synthetic phage display nanobody libraryA Amino acid frequencies per randomized position for the constructed library. Each pair of bars represents the experimental frequencies obtained by NGS and the corresponding theoretical (designed) frequencies. B, C Structural superposition of 33 and 37 Nb crystal structures showing 10 aa-long and 14 aa-long CDR3 loops, respectively. D Structural superposition of 100 randomly selected library Nbs modeled with NanoNetBack to article page