Table 1 Unique and novel essential genes in E. meningoseptica G4076

Two component system

 WP_016198590.1

Response regulator transcription factor

K07665

–

Cytoplasmic

 WP_016199055.1

LytTR family DNA-binding domain-containing protein

K07705

–

Cytoplasmic

 WP_016198441.1

Response regulator transcription factor

K07665

–

Cytoplasmic

 WP_016199099.1

LytTR family DNA-binding domain-containing protein

K07705

–

Cytoplasmic

 WP_016200043.1

VanW family protein

K18346

–

Unknown

 WP_016199769.1

Two-component sensor histidine kinase

K07636

2.7.13.3

Cytoplasmic membrane

Beta-lactam resistance

 WP_016170088.1

Penicillin-binding protein 2^a

K05515

3.4.16.4

Cytoplasmic membrane

 WP_016170028.1

Transglycosylase domain-containing protein^a

K05366

2.4.1.129

Cytoplasmic membrane

DNA replication

 WP_016169884.1

Ribonuclease HI

K03469

3.1.26.4

Unknown

 WP_016199473.1

DNA primase

K02316

2.7.7.101

Cytoplasmic

 WP_019051072.1

DNA polymerase III subunit gamma/tau^a

K02343

2.7.7.7

Cytoplasmic

 WP_016198779.1

DNA polymerase III subunit delta\′

K02341

2.7.7.7

Cytoplasmic

Homologous recombination

 WP_016199810.1

Holliday junction branch migration protein RuvA

K03550

3.6.4.12

Cytoplasmic

 WP_016198560.1

Holliday junction branch migration DNA helicase RuvB

K03551

3.6.4.12

Cytoplasmic

 WP_016200627.1

Recombination protein RecR

K06187

–

Cytoplasmic

 WP_016199534.1

DNA replication and repair protein RecF

K03629

–

Translation

 WP_009085459.1

30S ribosomal protein S2^a

K02967

–

Cytoplasmic

 WP_016200426.1

30S ribosomal protein S9^a

K02996

–

Cytoplasmic

 WP_009087380.1

30S ribosomal protein S12^a

K02946

–

Cytoplasmic

 WP_009087378.1

30S ribosomal protein S7^a

K02992

–

Cytoplasmic

 WP_016197802.1

30S ribosomal protein S10^a

K02946

–

Cytoplasmic

 WP_009087341.1

50S ribosomal protein L4^a

K02926

–

Cytoplasmic

 WP_016197785.1

50S ribosomal protein L16^a

K02878

–

Cytoplasmic

 WP_009087327.1

50S ribosomal protein L14

K02874

–

Cytoplasmic

 WP_016197784.1

50S ribosomal protein L24

K02895

–

Cytoplasmic

 WP_009087314.1

30S ribosomal protein S5^a

K02988

–

Cytoplasmic

 WP_016197779.1

50S ribosomal protein L15

K02876

–

Cytoplasmic

 WP_016197776.1

30S ribosomal protein S13^a

K02952

–

Cytoplasmic

 WP_009087288.1

30S ribosomal protein S11^a

K02948

–

Cytoplasmic

 WP_016170211.1

30S ribosomal protein S4^a

K02986

–

Cytoplasmic

 WP_016170209.1

50S ribosomal protein L17

K02879

–

Cytoplasmic

 WP_016198862.1

30S ribosomal protein S16

K02959

–

Cytoplasmic

 WP_016200457.1

50S ribosomal protein L20

K02887

–

Cytoplasmic

 WP_016199408.1

30S ribosomal protein S1

K02945

–

Cytoplasmic

 WP_016200561.1

50S ribosomal protein L9

K02939

–

Cytoplasmic

ABC Transporters

 WP_016198610.1

ATP-binding cassette domain-containing protein^a

K09812

–

Cytoplasmic membrane

 WP_016198126.1

ABC transporter ATP-binding protein^a

K09810

7.6.2.-

Cytoplasmic membrane

Protein export

 WP_026149261.1

Signal peptidase I^a

K03100

3.4.21.89

Cytoplasmic membrane

Methane metabolism

 WP_016198134.1

Phosphoenolpyruvate carboxylase^a

K01595

4.1.1.31

Cytoplasmic

Base excision repair

 WP_016170024.1

Endonuclease III

K10773

4.2.99.18

Cytoplasmic

Biotin metabolism

 WP_016199146.1

Dethiobiotin synthase

K01935

6.3.3.3

Cytoplasmic