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Table 1 Primers used for Dirofilaria species identification

From: A study on canine dirofilariasis in selected areas of Sri Lanka

Primer pair

Primer sequence

Gene target

Product origin

Product size (base-pairs)

Reference

D.imm-F1

CATCAGGTGATGATGTGATGAT

ITS 2

D. immitis

302

[17]

D.imm-R1

TGATTGGATTTTAACGTATCATT

DIR 3

CCGGTAGACCATGGCATTAT

5S rRNA

D. repens

246

[18]

DIR 4

CGGTCTTGGACGTTTGGTTA

  1. ITS internal transcribed spacer, 5S rRNA ribosomal 5S ribonucleic acid.