Fig. 1

Motility of bactofilin knock-out mutants A Flagella mediated motility assayed on 0.3% LB-agar plates imaged after 24 h incubation at 30°C. B Colony growth of 3610 bactofilin mutants on 0.3% agar plates (upper-lane) after 6 h of incubation, 0.5% agar plates after 24 h (middle lane) incubation and on 1.5% agar after 24 h of incubation (third lane). C Same colony of 3610_ΔbacF on 0.3% agar after 6 h and 24 h incubation. Colonies frequently developed motility suppressor mutations between 8 and 24 h post inoculation. Cells from the center (indicated by a single asterisk) and the periphery (double asterisk) were comparatively streaked on a new 0.3% agar plate and incubated for 8 h