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Fig. 1 | BMC Research Notes

Fig. 1

From: Mouse mesoderm-specific transcript inhibits adipogenic differentiation and induces trans-differentiation into hepatocyte-like cells in 3T3-L1 preadiocytes

Fig. 1

Mest overexpression reduces lipid accumulation in 3T3-L1 cells. A The expression levels of Mest and 36B4 mRNA as housekeeping gene in 3T3-L1 parent cells (P), 3T3-L1 mock-transfected cells (mock), and 3T3-L1-Mest (Mest). B Western blot analysis of the expression of Mest and β-actin as internal control in 3T3-L1 parent cells (P), 3T3-L1 mock-transfected cells (mock), and 3T3-L1-Mest (Mest). The values under each lane indicated relative density of the band of Mest protein normalized to β-actin. C Representative images of lipid accumulation by Oil Red O staining in 3T3-L1 mock-transfected cells, and 3T3-L1-Mest (Mest). The differentiation was induced by treatment of a standard adipogenic cocktail and additional reagent, 10 μM pioglitazone, which promotes the differentiation and makes it easy to evaluate of lipid accumulation. D Representative photographs of 24-well cell culture plate of Oil Red O staining in undifferentiated 3T3-L1 cells (UD), mock-transfected cells (mock), and Mest-overexpressing cells (Mest). E Quantification of Oil Red O retention in undifferentiated (UD) and differentiated (Diff.) 3T3-L1 mock and 3T3-L1 Mest. Values are presented as the mean ± SD (n = 8). **P < 0.01 vs 3T3-L1 mocked cells

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