Fig. 2

Knockdown of Mest by siRNA enhances adipogenic differentiation of 3T3-L1 cells. A The expression levels of Mest and 36B4 mRNA as housekeeping gene in 3T3-L1 cells, which were transfected with siRNAs for Mest (siRNA 1 and 2) or with RNAi negative control. B Western blot analysis of the expression of Mest and β-actin as internal control in 3T3-L1 cells, which were transfected with siRNAs for Mest (siRNA 1 and 2) or with RNAi negative control. The values under bands indicated relative density of the bands of Mest protein normalized to β-actin. C Representative images and (D) quantification of lipid accumulation by Oil Red O staining in 3T3-L1 cells, treated with control RNA and siRNAs for Mest (siRNA 1 and 2). Values are presented as the mean ± SD (n = 5). **P < 0.01 vs control RNA treated-3T3-L1 cells