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Table 1 Summary table: Fluorescence increase analysis

From: Quantifying heterologous gene expression during ectopic MazF production in Escherichia coli

A. Fluorescence increase of mazF-induced cultures compared to the uninduced cultures % increase p-value
Leaderless gfpΔACA reporter
  High-copy plasmid, after 2 h 34.4 ± 2.9a 0.002
  High-copy plasmid, after 6 h 132.8 ± 17.9 0.006
  Low-copy plasmid, after 2 h − 0.1 ± 0.6 nsb
  Low-copy plasmid, after 6 h 17.1 ± 4.0 0.021
Canonical gfpΔACA reporter
  High-copy plasmid, after 2 h 203.0 ± 19.0 0.0003
  High-copy plasmid, after 6 h 79.4 ± 39.2 ns
  Low-copy plasmid, after 2 h 112.7 ± 14.0 0.004
  Low-copy plasmid, after 6 h 133.9 ± 20.2 0.001
B. Fluorescence increase during mazF overexpression: 6 h after induction compared to 2 h after induction % increase p-value
Leaderless gfpΔACA reporter
 High-copy plasmid 77.6 ± 13.4 0.01
 Low-copy plasmid 12.3 ± 6.6 ns
Canonical gfpΔACA reporter
 High-copy plasmid 123.9 ± 60.0 ns
 Low-copy plasmid 113.4 ± 5.6 1.3E−05
mCherry reporter
 Chromosomal 34.7 ± 27.9 0.001
  1. amean ± standard deviation
  2. bns stands for not significant