From: Quantifying heterologous gene expression during ectopic MazF production in Escherichia coli
A. Fluorescence increase of mazF-induced cultures compared to the uninduced cultures | % increase | p-value |
---|---|---|
Leaderless gfpΔACA reporter | ||
High-copy plasmid, after 2 h | 34.4 ± 2.9a | 0.002 |
High-copy plasmid, after 6 h | 132.8 ± 17.9 | 0.006 |
Low-copy plasmid, after 2 h | − 0.1 ± 0.6 | nsb |
Low-copy plasmid, after 6 h | 17.1 ± 4.0 | 0.021 |
Canonical gfpΔACA reporter | ||
High-copy plasmid, after 2 h | 203.0 ± 19.0 | 0.0003 |
High-copy plasmid, after 6 h | 79.4 ± 39.2 | ns |
Low-copy plasmid, after 2 h | 112.7 ± 14.0 | 0.004 |
Low-copy plasmid, after 6 h | 133.9 ± 20.2 | 0.001 |
B. Fluorescence increase during mazF overexpression: 6 h after induction compared to 2 h after induction | % increase | p-value |
---|---|---|
Leaderless gfpΔACA reporter | ||
High-copy plasmid | 77.6 ± 13.4 | 0.01 |
Low-copy plasmid | 12.3 ± 6.6 | ns |
Canonical gfpΔACA reporter | ||
High-copy plasmid | 123.9 ± 60.0 | ns |
Low-copy plasmid | 113.4 ± 5.6 | 1.3E−05 |
mCherry reporter | ||
Chromosomal | 34.7 ± 27.9 | 0.001 |