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Table 1 Summary table: Fluorescence increase analysis

From: Quantifying heterologous gene expression during ectopic MazF production in Escherichia coli

A. Fluorescence increase of mazF-induced cultures compared to the uninduced cultures

% increase

p-value

Leaderless gfpΔACA reporter

  High-copy plasmid, after 2 h

34.4 ± 2.9a

0.002

  High-copy plasmid, after 6 h

132.8 ± 17.9

0.006

  Low-copy plasmid, after 2 h

− 0.1 ± 0.6

nsb

  Low-copy plasmid, after 6 h

17.1 ± 4.0

0.021

Canonical gfpΔACA reporter

  High-copy plasmid, after 2 h

203.0 ± 19.0

0.0003

  High-copy plasmid, after 6 h

79.4 ± 39.2

ns

  Low-copy plasmid, after 2 h

112.7 ± 14.0

0.004

  Low-copy plasmid, after 6 h

133.9 ± 20.2

0.001

B. Fluorescence increase during mazF overexpression: 6 h after induction compared to 2 h after induction

% increase

p-value

Leaderless gfpΔACA reporter

 High-copy plasmid

77.6 ± 13.4

0.01

 Low-copy plasmid

12.3 ± 6.6

ns

Canonical gfpΔACA reporter

 High-copy plasmid

123.9 ± 60.0

ns

 Low-copy plasmid

113.4 ± 5.6

1.3E−05

mCherry reporter

 Chromosomal

34.7 ± 27.9

0.001

  1. amean ± standard deviation
  2. bns stands for not significant