Fig. 1

Expression of NMDAR1 and CB1R in the cerebellar cortex of C57BL/6 (C57) and BTBR + Itpr3tf/J (BT) mice raised in standard environments. A Fluorescent signal of NMDAR1 (red). B Images corresponding to the squares in A. C Images obtained from averaging the fluorescence of a Z-stack of 10 images separated by 1 µm. D images corresponding to the squares in C. The contours emphasize the shape of Purkinje cell somas and dendrites. E–H As in A–F for the expression of CB1 receptors (green) H. The contours emphasize the CB1R presynaptic terminals around Purkinje cell soma. ML, Molecular cell layer, PCL, Purkinje cell layer; GCL, Granule cell layer. All slices were stained with DAPI and identically processed (blue)