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Fig. 2 | BMC Research Notes

Fig. 2

From: Contribution of a novel gene to lysergic acid amide synthesis in Metarhizium brunneum

Fig. 2

Accumulation of LAH and other lysergic acid amides in wild-type M. brunneum versus an easP knockout strain. Extracts were prepared from larvae of the model insect Galleria mellonella seven days after infection with the indicated strains. Moles of ergot alkaloids were measured relative to a standard curve of ergonovine and normalized to mass of the fungus-unique sterol ergosterol as a surrogate for fungal biomass. Bars indicate means (n = 6 fungus-infected larvae), and error bars indicate standard error. Different letters within an alkaloid comparison indicate that fungal strain had a significant effect on accumulation of the indicated lysergic acid amide (P < 0.05)

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